Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40

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dc.contributor.advisor Shonhai, A.
dc.contributor.advisor Burger, A.
dc.contributor.author Mutavhatsindi, Hygon
dc.date.accessioned 2016-08-17T09:02:51Z
dc.date.available 2016-08-17T09:02:51Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/11602/626
dc.description MSc (Biochemistry)
dc.description Department of Biochemistry
dc.description.abstract Malaria is caused by protozoa of the genus Plasmodium. Malaria accounts for approximately more than half a million deaths yearly. Of the five species of Plasmodium, P. falciparum accounts for the most deadly form of the disease. P. falciparum survives under various physiological conditions during its life cycle. The parasite employs its molecular chaperones machinery particularly heat shock proteins (Hsps) to protect its protein constituents during physiological stress. Hsps are conserved molecules that constitute a major part of the cell’s molecular chaperone system. P. falciparum Hsps play an important cyto-protective role guaranteeing that the malarial parasite survives under the severe conditions that prevail in the host environment. PFF1010c is a type IV P. falciparum heat shock protein 40. PFF1010c is predicted to be expressed only at the gametocyte stage of the malarial parasite’s life cycle. The aim of the current study was to investigate the expression PFF1010c by parasites and the gametocyte stage as well as characterize the structure-function features of the protein. PFF1010c was successfully expressed in E. coli cells. Despite successful expression of the protein, its purification proved problematic. The attempt to purify PFF1010c was carried out under both native and denaturing conditions. Far Western blot analysis to investigate direct interaction between PFF1010c and PfHsp70-1 was conducted and no interaction was observed. Malarial parasites were harvested at different stages and total protein was isolated. The expression of PFF1010c was confirmed to occur at the gametocyte stage of the parasite’s development using Western blot analysis. This study confirmed that PFF1010c is only expressed at the gametocyte stage of the malarial parasite. Furthermore, PFF1010c was not expressed at the asexual stage. Possible interactors of PFF1010c were predicted by STRING, a bioinformatics based tool. The expression of PfHsp90, PfHop and PfHsp70-1 at the gametocyte stage was investigated and confirmed by Western blot analyses. en_US
dc.format.extent I online resource (xiv, 102 leaves, cor illustrations)
dc.language.iso en en_US
dc.rights University of Venda
dc.subject Malaria en_US
dc.subject Protozoa en_US
dc.subject Plasmodium falciparum en_US
dc.subject Protein en_US
dc.subject Heat en_US
dc.subject.ddc 614.532
dc.subject.lcsh Plasmodium falciparum
dc.subject.lcsh Malaria
dc.subject.lcsh Plasmoduum
dc.subject.lcsh Ptotozoan diseases
dc.title Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40 en_US
dc.type Dissertation en_US

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