Jideani, A. I. O.Kgatla, T. E.Mashau, M. E.Lekhuleni, Isobel Lerato Gosh2020-11-032020-11-032020-08-24Lekhuleni, Isobel Lerato Gosh (2020) Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel. University of Venda, South Africa.<http://hdl.handle.net/11602/1625>.http://hdl.handle.net/11602/1625MSCPNTDepartment of Food Science and TechnologyThe study aimed to determine the physicochemical properties, extract and characterise pectin from three varieties (green, purple and orange) of prickly pear (Opuntia spp) fruit peel. Research samples were collected from Bothlokwa Mphakane village in Limpopo province of South Africa. The experimental design for this study was randomized complete block design and each treatment was conducted in triplicate. Pectin was extracted using sulphuric acid at four different pH levels (1, 2, 3 & 4) and microwave power levels (low, medium & high). The characterisation of extracted pectin was done by calculating the ash, moisture, equivalent weight, anhydrouronic acid, methoxyl content, and degree of esterification. Functional groups present in extracted and commercial pectin were investigated for similarities using Fourier Transform Infrared Spectroscopy. The yield of pectin for purple ranged from 2.9% to 13.8%, orange from 1.4% to 9.8% and from 2.3% to 10.0% for green prickly pear peel. A decrease in pH resulted in an increased pectin yield and an increase in microwave power level increased the pectin yield. Maximum yields of 13.8% on purple, 10.0% on green and 9.8% on orange were obtained at pH 1.0 and medium power level. The best condition for extraction using sulphuric acid was at a medium power level and pH 1.0. The pectins obtained were compared against each other in terms of yield, physicochemical characteristics and chemical structure. The ash content of the extracted pectin was significantly higher at 25.16 ± 0.69, 34.26 ± 1.92 and 36.30 ± 1.07, however the pectin showed lower moisture content and equivalent weight. The methoxyl contents were 2.28 ± 0.26, 2.38 ± 0.21 and 3.86 ±0.31, anhydrouronic acid contents were 25.58 ± 2.03, 25.93 ± 2.35 and 38.84 ± 2.29, and degree of esterification were 49.87 ± 0.17, 50.63 ± 4.76 and 56.39 ± 1.60 across the orange, purple and green varieties, respectively. The prickly pear peel pectin spectra exhibited similarities in its absorption pattern to that of commercial citrus pectin. Therefore, the extraction of pectin from prickly pear peels is feasible in viewpoint of yield and quality, and that the pectin has potential for substitution of citrus pectin in the food processing industry.1 online resource (xi, 73 leaves : color illustrations)enUniversity of VendaPrickly pearUCTDPectinExtractionYieldFunctional group664.80413096825Pectin -- South Africa -- LimpopoPears -- South Africa -- LimpopoExtraction and characterization of pectin from prickly pear (Opuntia spp.) peelDissertationLekhuleni ILG. Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel. []. , 2020 [cited yyyy month dd]. Available from: http://hdl.handle.net/11602/1625Lekhuleni, I. L. G. (2020). <i>Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel</i>. (). . Retrieved from http://hdl.handle.net/11602/1625Lekhuleni, Isobel Lerato Gosh. <i>"Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel."</i> ., , 2020. http://hdl.handle.net/11602/1625TY - Dissertation AU - Lekhuleni, Isobel Lerato Gosh AB - The study aimed to determine the physicochemical properties, extract and characterise pectin from three varieties (green, purple and orange) of prickly pear (Opuntia spp) fruit peel. Research samples were collected from Bothlokwa Mphakane village in Limpopo province of South Africa. The experimental design for this study was randomized complete block design and each treatment was conducted in triplicate. Pectin was extracted using sulphuric acid at four different pH levels (1, 2, 3 & 4) and microwave power levels (low, medium & high). The characterisation of extracted pectin was done by calculating the ash, moisture, equivalent weight, anhydrouronic acid, methoxyl content, and degree of esterification. Functional groups present in extracted and commercial pectin were investigated for similarities using Fourier Transform Infrared Spectroscopy. The yield of pectin for purple ranged from 2.9% to 13.8%, orange from 1.4% to 9.8% and from 2.3% to 10.0% for green prickly pear peel. A decrease in pH resulted in an increased pectin yield and an increase in microwave power level increased the pectin yield. Maximum yields of 13.8% on purple, 10.0% on green and 9.8% on orange were obtained at pH 1.0 and medium power level. The best condition for extraction using sulphuric acid was at a medium power level and pH 1.0. The pectins obtained were compared against each other in terms of yield, physicochemical characteristics and chemical structure. The ash content of the extracted pectin was significantly higher at 25.16 ± 0.69, 34.26 ± 1.92 and 36.30 ± 1.07, however the pectin showed lower moisture content and equivalent weight. The methoxyl contents were 2.28 ± 0.26, 2.38 ± 0.21 and 3.86 ±0.31, anhydrouronic acid contents were 25.58 ± 2.03, 25.93 ± 2.35 and 38.84 ± 2.29, and degree of esterification were 49.87 ± 0.17, 50.63 ± 4.76 and 56.39 ± 1.60 across the orange, purple and green varieties, respectively. The prickly pear peel pectin spectra exhibited similarities in its absorption pattern to that of commercial citrus pectin. Therefore, the extraction of pectin from prickly pear peels is feasible in viewpoint of yield and quality, and that the pectin has potential for substitution of citrus pectin in the food processing industry. DA - 2020-08-24 DB - ResearchSpace DP - Univen KW - Prickly pear KW - Pectin KW - Extraction KW - Yield KW - Functional group LK - https://univendspace.univen.ac.za PY - 2020 T1 - Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel TI - Extraction and characterization of pectin from prickly pear (Opuntia spp.) peel UR - http://hdl.handle.net/11602/1625 ER -