Zininga, TawandaAnokwuru, Chineda P.Sigidi, Muendi T.Tshisikhawe, Milingoni P.Ramaite, Isaiah I. D.Traore, Afsatou N.Hoppe, HeinrichShonhai, AddmorePotgieter, Natasha2019-06-172019-06-172017Zininga, Tawanda, et al. (2017) Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function, University of Venda, South Africa. Molecules 2017, 22, 1224; doi:10.3390/molecules22071224.pp. 1-13.DOI:10.3390/molecules22071224http://hdl.handle.net/11602/1374Department of BotanyMalaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70) function. Heat shock protein 70 (Hsp70) are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function.enAntimalarial activityUCTDHsp70Molecular chaperonePterocarpus anglonesisZiziphus mucronataArticle© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Zininga T, Anokwuru Chineda P, Sigidi Muendi T, Tshisikhawe Milingoni P, Ramaite Isaiah I D, Traore Afsatou N, et al. Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function. 2017; http://hdl.handle.net/11602/1374.Zininga, T., Anokwuru, Chineda P., Sigidi, Muendi T., Tshisikhawe, Milingoni P., Ramaite, Isaiah I. D., Traore, Afsatou N., ... Potgieter, N. (2017). Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function. http://hdl.handle.net/11602/1374Zininga, Tawanda, Chineda P. Anokwuru, Muendi T. Sigidi, Milingoni P. Tshisikhawe, Isaiah I. D. Ramaite, Afsatou N. Traore, Heinrich Hoppe, Addmore Shonhai, and Natasha Potgieter "Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function." (2017) http://hdl.handle.net/11602/1374TY - Article AU - Zininga, Tawanda AU - Anokwuru, Chineda P. AU - Sigidi, Muendi T. AU - Tshisikhawe, Milingoni P. AU - Ramaite, Isaiah I. D. AU - Traore, Afsatou N. AU - Hoppe, Heinrich AU - Shonhai, Addmore AU - Potgieter, Natasha AB - Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70) function. Heat shock protein 70 (Hsp70) are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function. DA - 2017 DB - ResearchSpace DP - Univen KW - Antimalarial activity KW - Hsp70 KW - Molecular chaperone KW - Pterocarpus anglonesis KW - Ziziphus mucronata LK - https://univendspace.univen.ac.za PY - 2017 T1 - Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function TI - Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function UR - http://hdl.handle.net/11602/1374 ER -