2026-09-25T00:29:45Zhttps://univendspace.univen.ac.za/server/oai/request

oai:univendspace.univen.ac.za:11602/6322024-09-10T14:46:05Zcom_11602_1928com_11602_1914com_11602_1897com_11602_737col_11602_2140col_11602_738

Potgieter, Natasha Mojapelo, M. P. Sengani, David 2016-08-18T12:49:54Z 2016-08-18T12:49:54Z 2015 Sengani, D. 2015. Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa. . . http://hdl.handle.net/11602/632 http://hdl.handle.net/11602/632 Sengani D. Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa. []. , 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11602/632 Sengani, D. (2015). <i>Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa</i>. (). . Retrieved from http://hdl.handle.net/11602/632 Sengani, David. <i>"Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa."</i> ., , 2015. http://hdl.handle.net/11602/632 TY - Dissertation AU - Sengani, David AB - Polychlorinated biphenyls (PCBs) are worldwide environmental pollutants which contaminate the environment through careless disposal practices and accidental spills or leakages from electrical transformers. These organic compounds are lipophilic chemicals soluble in fats, slightly soluble in water and readily bioaccumulated in the fatty tissues of fish, birds, animals and humans. The main objective of this study was to isolate and identify PCB degrading bacteria from PCB contaminated soils and test them for their degradation ability of PCBs in natural habitat conditions. Three bacteria species which comprise of Gram negative and Gram positive microorganisms were isolated and identified through biochemical tests, catalase tests, oxidase tests and morphological study and included Burkholderia cepacia, Pasteurella pneumotropica and Enterococcus faecalis. The results indicated that, there was an overall decrease of PCB concentration level and the readings ranged between -1.51 and -1.79 respectively for all the microorganisms. Enterococcus faecalis remove as much as 32% of PCBs in the contaminated soil samples. Whereas Pasteurella pneumotropica could remove 24% of PCBs, Burkholderia cepacia 21% of PCBs and the mixed culture removed 23%. Data showed that the 3 bacterial strains could tolerate high concentration of PCBs. The results provided the evidence that naturally occurring bacteria in soil contaminated with PCBs have the potential to degrade PCBs. Statistical analysis showed that there was a significant positive correlation between bacteria growth and treatment with a coefficient of (r) =0.1459 and p value <0.001. DA - 2015 DB - ResearchSpace DP - Univen KW - Bacteria KW - Bioaccumulation KW - Biodegradation KW - Bioremediation KW - Polychorinate biphenyls KW - contaminated soils LK - https://univendspace.univen.ac.za PY - 2015 T1 - Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa TI - Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa UR - http://hdl.handle.net/11602/632 ER - MENVM Department of Ecology and Resource Management Polychlorinated biphenyls (PCBs) are worldwide environmental pollutants which contaminate the environment through careless disposal practices and accidental spills or leakages from electrical transformers. These organic compounds are lipophilic chemicals soluble in fats, slightly soluble in water and readily bioaccumulated in the fatty tissues of fish, birds, animals and humans. The main objective of this study was to isolate and identify PCB degrading bacteria from PCB contaminated soils and test them for their degradation ability of PCBs in natural habitat conditions. Three bacteria species which comprise of Gram negative and Gram positive microorganisms were isolated and identified through biochemical tests, catalase tests, oxidase tests and morphological study and included Burkholderia cepacia, Pasteurella pneumotropica and Enterococcus faecalis. The results indicated that, there was an overall decrease of PCB concentration level and the readings ranged between -1.51 and -1.79 respectively for all the microorganisms. Enterococcus faecalis remove as much as 32% of PCBs in the contaminated soil samples. Whereas Pasteurella pneumotropica could remove 24% of PCBs, Burkholderia cepacia 21% of PCBs and the mixed culture removed 23%. Data showed that the 3 bacterial strains could tolerate high concentration of PCBs. The results provided the evidence that naturally occurring bacteria in soil contaminated with PCBs have the potential to degrade PCBs. Statistical analysis showed that there was a significant positive correlation between bacteria growth and treatment with a coefficient of (r) =0.1459 and p value <0.001. 1 online resource (x, 75 leaves : color illustrations; color map) en Bacteria Bioaccumulation Biodegradation Bioremediation Polychorinate biphenyls contaminated soils UCTD 615.9512 Polychlorinated biphenyls Biphenyl compounds Organochlorine compounds Soil pollution Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa Dissertation open.access