2026-09-24T15:33:27Zhttps://univendspace.univen.ac.za/server/oai/requestoai:univendspace.univen.ac.za:11602/26182024-09-10T14:42:27Zcom_11602_1926com_11602_1914com_11602_1897com_11602_737col_11602_2146col_11602_738
Traore, A. N.
Potgieter, N.
Mavumengwane, V.
Marole, Tsireledzo
2023
2023-11-08T14:10:25Z
2023-11-08T14:10:25Z
2023-10-05
Marole, T. (2023). Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain. University of Venda, Thohoyandou, South Africa.<http://hdl.handle.net/11602/2618>.
http://hdl.handle.net/11602/2618
Marole T. Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain. []. , 2023 [cited yyyy month dd]. Available from: http://hdl.handle.net/11602/2618
Marole, T. (2023). <i>Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain</i>. (). . Retrieved from http://hdl.handle.net/11602/2618
Marole, Tsireledzo. <i>"Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain."</i> ., , 2023. http://hdl.handle.net/11602/2618
TY - Dissertation
AU - Marole, Tsireledzo
AB - Background: Tuberculosis is a major public health concern with over 2 billion people currently infected,
8.6 million cases per year and more than 1.3 million deaths annually. Tuberculosis is an infectious
disease caused by Mycobacterium tuberculosis (MTB) which mainly affects the lungs causing
pulmonary TB. In South Africa, the use of the Directly Observed Treatment Short Course (DOTS)
strategy is applied where patients have to travel to primary Health care facilities (DOTS clinics) to get
their medication. The rising prevalence of multidrug resistant and extensive drug resistant TB
throughout the world highlights the critical need for novel anti-tuberculosis compounds/drugs.
Objective: The aim of this study was to determine the cytotoxicity and antimycobacterial activities of
Sand Olive and Marula.
Methodology: The leaves (L) and barks (B) of Dodonaea Viscosa Anguistifolia (DVA or D) and S.
birrea (S) plants were collected in the Vhembe district, Limpopo. Separate macerations in methanol (C)
and distilled water (H) were done to obtain a total of 8 crude extracts (SBC, SBH, SLC. SLH, DBC,
DBH, DLC, DLH). Phytochemical analysis was conducted on all extracts using thin layer
chromatography (TLC), the profiling of metabolites was achieved with liquid chromatography-Mass
spectrometry (LCMS) method. The antimycobacterial activity against M. smegmatis was determined
using the microbroth dilution assay. The cytotoxicity of the plant extracts was assessed using the MTT
assay.
Results: The percentage yield ranged from 21.07% (DBC) to 4.4% (DBH). The phytochemical
screening showed the presence of saponins, cardiac glycosides, steroids, terpenoids and tannins. The
BEA solvent system revealed more bands than the CEF solvent system while the EMW solvent system
was the least efficient. LCMS method showed sufficient revolving power to separate isomeric forms of
several compounds. From the extracts of DVA and S. birrea, 508 chemicals were present in all the
chromatograms assessed, but only 40 compounds were putatively identified and comprised of
flavonoids, phenolics, terpenoids, and saponins. Of the 8 plant extracts that were tested 5 (DLH
(50μg/ml (83.5%), (100μg/ml (85.9%)), DBH (50μg/ml (70.9%), (100μg/ml (60.3%)), DBC (50μg/ml
(76.6%), 100μg/ml (83.9%) SLH (50μg/ml (66.8%), (100μg/ml (66.1%) and SLC (50μg/ml (79.9%),
(100μg/ml (77.1%)) were found to have moderate cytotoxic effects on Vero cells at both treatment
concentration while 3 (DLC (50μg/ml (91.5%), (100μg/ml (105%), SBH (50μg/ml (98.7%), (100μg/ml
(106.8%) and SBC (50μg/ml (105.3%), (100μg/ml (118.2)) did increase viability of Vero cells. Amongst
all the samples screened for antimycobacterial activity, only 3 plant extracts (SBC, DBC and DBH)
inhibited the growth of M. smegmatis.
Conclusion: The results of the study demonstrate the potential use of DVA and S. birrea as alternative
treatment for tuberculosis. The bark of the plants may contain active compounds with anti-TB activities
needing further investigation.
DA - 2023-10-05
DB - ResearchSpace
DP - Univen
KW - Anti-mycobacterial
KW - Cytotoxicity
KW - Dodonae Viscosa Anguistifolia
KW - LCMS
KW - MTT
KW - M. snegmatis
KW - Sclerocarya Birrea
LK - https://univendspace.univen.ac.za
PY - 2023
T1 - Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain
TI - Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain
UR - http://hdl.handle.net/11602/2618
ER -
MSc (Microbioogy)
Department of Biochemistry and Microbiology
Background: Tuberculosis is a major public health concern with over 2 billion people currently infected,
8.6 million cases per year and more than 1.3 million deaths annually. Tuberculosis is an infectious
disease caused by Mycobacterium tuberculosis (MTB) which mainly affects the lungs causing
pulmonary TB. In South Africa, the use of the Directly Observed Treatment Short Course (DOTS)
strategy is applied where patients have to travel to primary Health care facilities (DOTS clinics) to get
their medication. The rising prevalence of multidrug resistant and extensive drug resistant TB
throughout the world highlights the critical need for novel anti-tuberculosis compounds/drugs.
Objective: The aim of this study was to determine the cytotoxicity and antimycobacterial activities of
Sand Olive and Marula.
Methodology: The leaves (L) and barks (B) of Dodonaea Viscosa Anguistifolia (DVA or D) and S.
birrea (S) plants were collected in the Vhembe district, Limpopo. Separate macerations in methanol (C)
and distilled water (H) were done to obtain a total of 8 crude extracts (SBC, SBH, SLC. SLH, DBC,
DBH, DLC, DLH). Phytochemical analysis was conducted on all extracts using thin layer
chromatography (TLC), the profiling of metabolites was achieved with liquid chromatography-Mass
spectrometry (LCMS) method. The antimycobacterial activity against M. smegmatis was determined
using the microbroth dilution assay. The cytotoxicity of the plant extracts was assessed using the MTT
assay.
Results: The percentage yield ranged from 21.07% (DBC) to 4.4% (DBH). The phytochemical
screening showed the presence of saponins, cardiac glycosides, steroids, terpenoids and tannins. The
BEA solvent system revealed more bands than the CEF solvent system while the EMW solvent system
was the least efficient. LCMS method showed sufficient revolving power to separate isomeric forms of
several compounds. From the extracts of DVA and S. birrea, 508 chemicals were present in all the
chromatograms assessed, but only 40 compounds were putatively identified and comprised of
flavonoids, phenolics, terpenoids, and saponins. Of the 8 plant extracts that were tested 5 (DLH
(50μg/ml (83.5%), (100μg/ml (85.9%)), DBH (50μg/ml (70.9%), (100μg/ml (60.3%)), DBC (50μg/ml
(76.6%), 100μg/ml (83.9%) SLH (50μg/ml (66.8%), (100μg/ml (66.1%) and SLC (50μg/ml (79.9%),
(100μg/ml (77.1%)) were found to have moderate cytotoxic effects on Vero cells at both treatment
concentration while 3 (DLC (50μg/ml (91.5%), (100μg/ml (105%), SBH (50μg/ml (98.7%), (100μg/ml
(106.8%) and SBC (50μg/ml (105.3%), (100μg/ml (118.2)) did increase viability of Vero cells. Amongst
all the samples screened for antimycobacterial activity, only 3 plant extracts (SBC, DBC and DBH)
inhibited the growth of M. smegmatis.
Conclusion: The results of the study demonstrate the potential use of DVA and S. birrea as alternative
treatment for tuberculosis. The bark of the plants may contain active compounds with anti-TB activities
needing further investigation.
National Research Foundation (NRF)
1 online resource (xi, 73 leaves): color illustrations, color maps
en
PDF
University of Venda
Anti-mycobacterial
UCTD
Cytotoxicity
Dodonae Viscosa Anguistifolia
LCMS
MTT
M. snegmatis
Sclerocarya Birrea
333.9532
Plant diversity
Plants
Plant species
Cytotoxicity and anti-mycobacterial activities of sclerocarya birrea and Dodonae viscosa anguistifolia against MTB strain
Dissertation
open.access