2026-09-24T11:42:20Zhttps://univendspace.univen.ac.za/server/oai/requestoai:univendspace.univen.ac.za:11602/14602024-09-10T14:39:53Zcom_11602_1924com_11602_1914com_11602_1897com_11602_737col_11602_2142col_11602_738
Mnyakeni-Moleele, S. S.
Tselanyane, M.
Maluleke, Khanyisa Amanda
2019
2019-10-15T10:22:04Z
2019-10-15T10:22:04Z
2019-08-20
Maluleke, Khanyisa Amanda (2019) Isolation and characterization of antidiabetic constituents of Bridelia Micrantha, University of Venda, South Africa.<http://hdl.handle.net/11602/1460>.
http://hdl.handle.net/11602/1460
Maluleke KA. Isolation and characterization of antidiabetic constituents of Bridelia Micrantha. []. , 2019 [cited yyyy month dd]. Available from: http://hdl.handle.net/11602/1460
Maluleke, K. A. (2019). <i>Isolation and characterization of antidiabetic constituents of Bridelia Micrantha</i>. (). . Retrieved from http://hdl.handle.net/11602/1460
Maluleke, Khanyisa Amanda. <i>"Isolation and characterization of antidiabetic constituents of Bridelia Micrantha."</i> ., , 2019. http://hdl.handle.net/11602/1460
TY - Dissertation
AU - Maluleke, Khanyisa Amanda
AB - Bridelia micrantha (Hochst) Baill (B. micrantha) is a South African medicinal plant used by traditional healers in the treatment of different human ailments including diabetes, gastrointestinal ailments, joint aches, cough, conjunctivitis, skin problems and malaria. Previous studies have demonstrated the antidiabetic activities of B. micrantha crude extracts in in vivo studies. However, there are no studies on the compounds responsible for the antidiabetic activity of the plant. The purpose of this study was to isolate and characterize the antidiabetic constituents from B. micrantha.
Materials and methods
Crude methanolic extracts of root, stem and leaf were investigated using in vitro antidiabetic enzyme assays. Antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The isolation of the antidiabetic constituents was carried out using column chromatography on silica gel. Purification of the active fraction was carried out using preparative thin layer chromatography (pTLC). Structure elucidation of the compound was carried out using Nuclear Magnetic Resonance (NMR) spectroscopy and by comparison with literature.
Results
The results obtained in this study indicated that root, stem and leaf extracts exhibited high inhibition activity against α-glucosidase (98.52, 98.62 and 81.62% respectively). A moderate inhibition against α-amylase enzyme was observed for root (65.62%) and stem (61.86%) extracts. Leaf fraction LFR5 exhibited a high inhibition activity of 96.19% against α-glucosidase. Moreover, the isolated compound showed 96.74% inhibition against α-glucosidase. DPPH results revealed that antioxidant activity of crude extracts was not significantly different and they were concentration-dependent. Reducing power results revealed that stem (119.31 μg/mL) extract had higher activities compared to root (125.17 μg/mL) and leaf (291.88 μg/mL) extracts.
Conclusion
Quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside was successfully isolated from B. micrantha leaves. Furthermore, quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside demonstrated the ability to inhibit significantly the carbohydrate hydrolysing enzyme α-glucosidase and therefore validate the ethnomedicinal use of B. micrantha in the management of diabetes
DA - 2019-08-20
DB - ResearchSpace
DP - Univen
KW - Diabetes mellitus (DM)
KW - Bridelia micrantha (Hochst)
KW - Medicinal plant
KW - Traditional healers
LK - https://univendspace.univen.ac.za
PY - 2019
T1 - Isolation and characterization of antidiabetic constituents of Bridelia Micrantha
TI - Isolation and characterization of antidiabetic constituents of Bridelia Micrantha
UR - http://hdl.handle.net/11602/1460
ER -
MSc (Chemistry)
Department of Chemistry
Bridelia micrantha (Hochst) Baill (B. micrantha) is a South African medicinal plant used by traditional healers in the treatment of different human ailments including diabetes, gastrointestinal ailments, joint aches, cough, conjunctivitis, skin problems and malaria. Previous studies have demonstrated the antidiabetic activities of B. micrantha crude extracts in in vivo studies. However, there are no studies on the compounds responsible for the antidiabetic activity of the plant. The purpose of this study was to isolate and characterize the antidiabetic constituents from B. micrantha.
Materials and methods
Crude methanolic extracts of root, stem and leaf were investigated using in vitro antidiabetic enzyme assays. Antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The isolation of the antidiabetic constituents was carried out using column chromatography on silica gel. Purification of the active fraction was carried out using preparative thin layer chromatography (pTLC). Structure elucidation of the compound was carried out using Nuclear Magnetic Resonance (NMR) spectroscopy and by comparison with literature.
Results
The results obtained in this study indicated that root, stem and leaf extracts exhibited high inhibition activity against α-glucosidase (98.52, 98.62 and 81.62% respectively). A moderate inhibition against α-amylase enzyme was observed for root (65.62%) and stem (61.86%) extracts. Leaf fraction LFR5 exhibited a high inhibition activity of 96.19% against α-glucosidase. Moreover, the isolated compound showed 96.74% inhibition against α-glucosidase. DPPH results revealed that antioxidant activity of crude extracts was not significantly different and they were concentration-dependent. Reducing power results revealed that stem (119.31 μg/mL) extract had higher activities compared to root (125.17 μg/mL) and leaf (291.88 μg/mL) extracts.
Conclusion
Quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside was successfully isolated from B. micrantha leaves. Furthermore, quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside demonstrated the ability to inhibit significantly the carbohydrate hydrolysing enzyme α-glucosidase and therefore validate the ethnomedicinal use of B. micrantha in the management of diabetes
Bridelia micrantha (Hochst) Baill (B. micrantha) is a South African medicinal plant used by traditional healers in the treatment of different human ailments including diabetes, gastrointestinal ailments, joint aches, cough, conjunctivitis, skin problems and malaria. Previous studies have demonstrated the antidiabetic activities of B. micrantha crude extracts in in vivo studies. However, there are no studies on the compounds responsible for the antidiabetic activity of the plant. The purpose of this study was to isolate and characterize the antidiabetic constituents from B. micrantha.
Materials and methods
Crude methanolic extracts of root, stem and leaf were investigated using in vitro antidiabetic enzyme assays. Antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The isolation of the antidiabetic constituents was carried out using column chromatography on silica gel. Purification of the active fraction was carried out using preparative thin layer chromatography (pTLC). Structure elucidation of the compound was carried out using Nuclear Magnetic Resonance (NMR) spectroscopy and by comparison with literature.
Results
The results obtained in this study indicated that root, stem and leaf extracts exhibited high inhibition activity against α-glucosidase (98.52, 98.62 and 81.62% respectively). A moderate inhibition against α-amylase enzyme was observed for root (65.62%) and stem (61.86%) extracts. Leaf fraction LFR5 exhibited a high inhibition activity of 96.19% against α-glucosidase. Moreover, the isolated compound showed 96.74% inhibition against α-glucosidase. DPPH results revealed that antioxidant activity of crude extracts was not significantly different and they were concentration-dependent. Reducing power results revealed that stem (119.31 μg/mL) extract had higher activities compared to root (125.17 μg/mL) and leaf (291.88 μg/mL) extracts.
Conclusion
Quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside was successfully isolated from B. micrantha leaves. Furthermore, quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside demonstrated the ability to inhibit significantly the carbohydrate hydrolysing enzyme α-glucosidase and therefore validate the ethnomedicinal use of B. micrantha in the management of diabetes.
1 online resource ()
en
University of Venda
Diabetes mellitus (DM)
Bridelia micrantha (Hochst)
Medicinal plant
Traditional healers
UCTD
Isolation and characterization of antidiabetic constituents of Bridelia Micrantha
Dissertation
open.access