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Ogola, J. B. O. Odindo, A. O. Vilakazi, Busisiwe 2018 2018-05-25T13:22:26Z 2018-05-25T13:22:26Z 2018-05-18 Vilakazi, B. 2018. The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour. . . http://hdl.handle.net/11602/1086 http://hdl.handle.net/11602/1086 Vilakazi B. The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour. []. , 2018 [cited yyyy month dd]. Available from: http://hdl.handle.net/11602/1086 Vilakazi, B. (2018). <i>The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour</i>. (). . Retrieved from http://hdl.handle.net/11602/1086 Vilakazi, Busisiwe. <i>"The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour."</i> ., , 2018. http://hdl.handle.net/11602/1086 TY - Dissertation AU - Vilakazi, Busisiwe AB - Chickpea (Cicer arietinum L.) is an excellent utilizer of residual soil moisture in agricultural ecosystems. However, its seed quality and hence reproduction is constrained by water stress, seed size and storage conditions. This study was carried out at the University of KwaZulu- Natal (UKZN), Pietermaritzburg Campus. It was conducted to evaluate the performance of chickpea genotypes (Desi-K, Saina-K and ICCV-K) with different seed sizes on seedling emergence (i), seed ageing effect on seed quality and imbibition of genotypes produced under water stressed and non-stressed conditions (ii), and (iii) the effect of water stress during seed development on sugars and protein accumulation, germination and seed vigour. Pot experiments were conducted under glasshouse / tunnel conditions at the Controlled Environment Facilities (CEF). The experiment for objective 1 was laid out as a single factor in completely randomized design (CRD). Data on emergence rate, final hypocotyl and complete emergence was collected. The small seeded Desi-K showed higher and faster emergence compared to medium sized Saina-K and large seeded ICCV-K. In the experiment of the second objective, seeds of the three genotypes were first obtained by production under water stressed and non-stressed growing conditions. They were then aged for 0, 1, 3, 5, or 7 days at 41 ºC and 100% relative humidity to form a 2 x 3 x 5 (water levels x genotypes x ageing) factorial design. Data was collected on germination percentage (GP), mean germination time (MGT), electrical conductivity (EC), tetrazolium chloride test (TZ) and imbibition weight. Seed ageing caused progressive loss of seed viability and vigour in all genotypes, which resulted in lower GP, delayed MGT, reduced TZ staining, cell death and high solute leakage from the seeds produced under the two water regimes. However, the effect was more severe under water stressed conditions. In the experiment for objective 3, seeds of all three genotypes were larger when grown under non-stressed condition compared to those under water stressed condition. These larger seeds had higher seed viability and germination percentage but lower electrical conductivity and mean germination time. Stressed seeds had higher soluble sugars than non-stressed seeds. It was deduced that irrigation during seed development reduces the final sugars and protein content but increases the seed size and physiological quality parameters allied to production of chickpea. Therefore, water provision to chickpea crop is critical during seed development. DA - 2018-05-18 DB - ResearchSpace DP - Univen KW - Chickpea KW - Seed quality KW - Water stress KW - Storage conditions KW - Seed size KW - Sugars LK - https://univendspace.univen.ac.za PY - 2018 T1 - The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour TI - The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour UR - http://hdl.handle.net/11602/1086 ER - MSCAGR (Plant Production) Department of Plant Production Chickpea (Cicer arietinum L.) is an excellent utilizer of residual soil moisture in agricultural ecosystems. However, its seed quality and hence reproduction is constrained by water stress, seed size and storage conditions. This study was carried out at the University of KwaZulu- Natal (UKZN), Pietermaritzburg Campus. It was conducted to evaluate the performance of chickpea genotypes (Desi-K, Saina-K and ICCV-K) with different seed sizes on seedling emergence (i), seed ageing effect on seed quality and imbibition of genotypes produced under water stressed and non-stressed conditions (ii), and (iii) the effect of water stress during seed development on sugars and protein accumulation, germination and seed vigour. Pot experiments were conducted under glasshouse / tunnel conditions at the Controlled Environment Facilities (CEF). The experiment for objective 1 was laid out as a single factor in completely randomized design (CRD). Data on emergence rate, final hypocotyl and complete emergence was collected. The small seeded Desi-K showed higher and faster emergence compared to medium sized Saina-K and large seeded ICCV-K. In the experiment of the second objective, seeds of the three genotypes were first obtained by production under water stressed and non-stressed growing conditions. They were then aged for 0, 1, 3, 5, or 7 days at 41 ºC and 100% relative humidity to form a 2 x 3 x 5 (water levels x genotypes x ageing) factorial design. Data was collected on germination percentage (GP), mean germination time (MGT), electrical conductivity (EC), tetrazolium chloride test (TZ) and imbibition weight. Seed ageing caused progressive loss of seed viability and vigour in all genotypes, which resulted in lower GP, delayed MGT, reduced TZ staining, cell death and high solute leakage from the seeds produced under the two water regimes. However, the effect was more severe under water stressed conditions. In the experiment for objective 3, seeds of all three genotypes were larger when grown under non-stressed condition compared to those under water stressed condition. These larger seeds had higher seed viability and germination percentage but lower electrical conductivity and mean germination time. Stressed seeds had higher soluble sugars than non-stressed seeds. It was deduced that irrigation during seed development reduces the final sugars and protein content but increases the seed size and physiological quality parameters allied to production of chickpea. Therefore, water provision to chickpea crop is critical during seed development. NRF 1 online resource (xi, 84 leaves : color illustrations) en University of Venda Chickpea Seed quality Water stress Storage conditions Seed size Sugars UCTD 633.370968475 Water -- South Africa -- KwaZulu - Natal Chickpea -- South Africa -- KwaZulu - Natal Crops -- Drought tolerance -- South Africa -- KwaZulu - Natal Crops -- Effect of stress on -- South Africa -- KwaZulu - Natal Crops -- Effect of temperature on -- South Africa -- KwaZulu - Natal Cicer -- South Africa -- KwaZulu - Natal Legumes -- South Africa -- KwaZulu - Natal Seeds -- Quality The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour Dissertation open.access