Identification of human papilloma virus, hepatitis B virus and human herpes virus type 8 in plasma of benign prostatic hyperplasia and prostate cancer patients in South Africa

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dc.contributor.advisor Bessong, Pascal Obong
dc.contributor.advisor Borman, Riana
dc.contributor.author Munzhedzi, Mukhethwa
dc.date.accessioned 2017-06-08T14:13:49Z
dc.date.available 2017-06-08T14:13:49Z
dc.date.issued 2017-05
dc.identifier.uri http://hdl.handle.net/11602/673
dc.description MSc (Microbiology)
dc.description Department of Microbiology
dc.description.abstract Background: Prostate cancer (PCA) is a major health concern in males, particularly those above 40 years old. It is the most common form of cancer in males worldwide, including South Africa. In South Africa, the rate of histologically diagnosed prostate cancer is 40 per 100 000 in whites and 14 per 100 000 in blacks, and 1 in 8 men will develop PCA in their lifetime. Several reports have suggested the association of viruses in the pathogenesis of prostate cancer. Objectives: This study was aimed at identifying Hepatitis B virus (HBV), human papilloma virus (HPV) and human herpes virus type 8 (HHV-8), implicated in other forms of cancer, in a cohort of South African patients with either PCA or benign prostatic hyperplasia (BPH); and to seek possible associations thereof. Methods: The study group comprised 187 male patients recruited from Polokwane Hospital presenting with either PCA (staged by Gleason scores) or BPH. Enzyme-linked immunosorbent assay was used to detect antibodies to HHV-8 and HPV; and to detect hepatitis B surface antigen (HBsAg) in the plasma of the study subjects. Total DNA was extracted from plasma and targeted for the identification of HBV and HHV-8 DNA by nested PCR protocols. The HBV nested PCR protocol amplifies a 336bp fragment of the overlapping surface polymerase gene of HBV. The HHV-8 nested protocol amplifies a 233bp fragment of the ORF 26 gene of HHV-8. Amplified DNA products were purified, sequenced by the Sanger protocol and phylogenetically analysed for viral genotypes. The Chi-square test was used to infer statistically significant differences in the level of detection of viruses and the stage of prostate cancer development. Results: Of the 187 participants, a seroprevalence of 4.8% (9/187, HBsAg), 5.3% (10/187, HPV IgG antibody) and 27% (33/124, HHV-8 IgG antibody) were observed. HBsAg was detected more in individuals with BPH than those without and this was statistically significant at ( 2=6.0, p< 0.05). HHV-8 DNA was detected more in individuals in the 60-79 years age range and this was statistically significant at ( 2=61.1, p< 0.05). Occult HBV infection (that is the presence of HBV DNA in the absence of HBsAg) was detected in 23/178 (12.9%) of patients. Taking into account occult HBV infection, the overall prevalence of HBV was 17.7%. HBV genotype E was more prevalent (86.7%) followed by genotype A (13.3%). HHV-8 genotypes K and R were inferred. Apparently, this is the first report on the identification of HHV-8 genotypes K and R from South Africa. Conclusion: The current study has demonstrated for the first time, the presence of genotypes K and R of HHV-8 in South Africa. This study also suggests that there is a high level of occult genotype E HBV infection. Future studies will explore the virome in prostate cancer biopsies. en_US
dc.format.extent 1 online resource (xiii, 75 leaves : color illustrations)
dc.language.iso en en_US
dc.rights University of Venda
dc.subject HPV en_US
dc.subject HBV en_US
dc.subject HHV-8 en_US
dc.subject Prostate Cancer en_US
dc.subject Benign prostatic hyperplasia en_US
dc.subject.ddc 616.994630968
dc.subject.ddc Prostate -- Cancer -- South Africa
dc.subject.ddc Prostate -- Diagnosis -- South Africa
dc.subject.ddc Prostate -- Surgery -- South Africa
dc.subject.ddc Hepatitis B virus -- South Africa
dc.subject.ddc Hepatitis viruses -- South Africa
dc.subject.ddc Cancer in men -- South Africa
dc.title Identification of human papilloma virus, hepatitis B virus and human herpes virus type 8 in plasma of benign prostatic hyperplasia and prostate cancer patients in South Africa en_US
dc.type Dissertation en_US

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