Serologic and genotypic characterization of hepatitis B virus in HIV-1 infected patients from South West and Littoral Regions of Cameroon

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dc.contributor.author Magoro, Tshifhiwa
dc.contributor.author Gachara, George
dc.contributor.author Mavhandu, Lufuno
dc.contributor.author Lum, Emmaculate
dc.contributor.author Kimbi, Helen K.
dc.contributor.author Ndip, Roland N
dc.contributor.author Bessong, Pascal Obong
dc.date.accessioned 2016-10-31T10:16:31Z
dc.date.available 2016-10-31T10:16:31Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/11602/662
dc.description.abstract Background: HBV and HIV share similar transmission routes. Concurrent infection with the two viruses usually results in more severe and progressive liver disease, and a higher incidence of cirrhosis, liver cancer and mortality. Further, this co-infection may lead to cross-resistance between HIV and HBV drugs and increased liver injury, either due to direct hepatotoxicity or drug-related immune-reconstitution hepatitis. These challenges necessitate continuous surveillance for HBV among HIV infected individuals to guide patient management. We conducted this study to understand the serologic and genotypic characteristics of HBV among HIV/HBV infected patients in South West and Littoral Regions of Cameroon. Methods: Plasma samples were screened for HBsAg, HBeAg, Anti-HBs and anti-HBc using ELISA followed by DNA extraction from all HBsAg positive samples. A 366 bp region covering the overlapping surface/polymerase gene was amplified by a nested PCR and the product sequenced using Big Dye sequencing chemistry. The resulting sequences were then analyzed for genotypes and both escape and drug resistance mutations. Results: Of the 455 samples in this study, 25.5 % (n = 116) were HBsAg positive and 46 of these had their DNA successfully amplified. Genotype E was found in 32 samples (69.6 %) and genotype A in the rest of the samples. Escape mutations associated with failure of diagnosis (Y100C, R122K and Q129H) and with vaccine escape (Q129R and T131N) were detected in varying frequencies in the population. Polymerase mutations implicated in resistance to lamivudine and other ʟ-nucleoside analogues were detected in seven patients (15.2 %), while all the samples lacked mutations associated with resistance to adefovir and tenofovir. Conclusions: These findings suggest the endemicity of HBV and the predominance of genotypes A and E in the study population. Also, drug resistance findings support the use of tenofovir based ART regimens among HIV/HBV co-infected persons. There is need for continuous HBV screening and monitoring in HIV infected individuals in these regions en_US
dc.description.sponsorship Funded by HIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.subject Hepatitis B virus en_US
dc.subject HIV/HBV co-infection en_US
dc.subject HBV genotypes en_US
dc.subject Phylogenetic analysis en_US
dc.title Serologic and genotypic characterization of hepatitis B virus in HIV-1 infected patients from South West and Littoral Regions of Cameroon en_US
dc.type Article en_US
dc.description.version Publisher's version
dc.rights.holder Author's retain copyright

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