Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa

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dc.contributor.advisor Potgieter, Natasha
dc.contributor.advisor Mojapelo, M. P.
dc.contributor.author Sengani, David
dc.date.accessioned 2016-08-18T12:49:54Z
dc.date.available 2016-08-18T12:49:54Z
dc.date.issued 2015
dc.identifier.uri http://hdl.handle.net/11602/632
dc.description MENVM
dc.description Department of Ecology and Resource Management
dc.description.abstract Polychlorinated biphenyls (PCBs) are worldwide environmental pollutants which contaminate the environment through careless disposal practices and accidental spills or leakages from electrical transformers. These organic compounds are lipophilic chemicals soluble in fats, slightly soluble in water and readily bioaccumulated in the fatty tissues of fish, birds, animals and humans. The main objective of this study was to isolate and identify PCB degrading bacteria from PCB contaminated soils and test them for their degradation ability of PCBs in natural habitat conditions. Three bacteria species which comprise of Gram negative and Gram positive microorganisms were isolated and identified through biochemical tests, catalase tests, oxidase tests and morphological study and included Burkholderia cepacia, Pasteurella pneumotropica and Enterococcus faecalis. The results indicated that, there was an overall decrease of PCB concentration level and the readings ranged between -1.51 and -1.79 respectively for all the microorganisms. Enterococcus faecalis remove as much as 32% of PCBs in the contaminated soil samples. Whereas Pasteurella pneumotropica could remove 24% of PCBs, Burkholderia cepacia 21% of PCBs and the mixed culture removed 23%. Data showed that the 3 bacterial strains could tolerate high concentration of PCBs. The results provided the evidence that naturally occurring bacteria in soil contaminated with PCBs have the potential to degrade PCBs. Statistical analysis showed that there was a significant positive correlation between bacteria growth and treatment with a coefficient of (r) =0.1459 and p value <0.001. en_US
dc.format.extent 1 online resource (x, 75 leaves : color illustrations; color map)
dc.language.iso en en_US
dc.subject Bacteria en_US
dc.subject Bioaccumulation en_US
dc.subject Biodegradation en_US
dc.subject Bioremediation en_US
dc.subject Polychorinate biphenyls en_US
dc.subject contaminated soils en_US
dc.subject.ddc 615.9512
dc.subject.lcsh Polychlorinated biphenyls
dc.subject.lcsh Biphenyl compounds
dc.subject.lcsh Organochlorine compounds
dc.subject.lcsh Soil pollution
dc.title Bioremediation of polychlorinated biphenyls (PCBs) on contaminated soils : a case study of Rietvlei Farm Borehole No. 11, Limpopo Province, South Africa en_US
dc.type Dissertation en_US

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