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Isolation and structure elucidation of bioctive compounds from Rauvolfia Caffra Sond

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dc.contributor.advisor Ramaite, IDI
dc.contributor.advisor van Ree, T.
dc.contributor.author Tlhapi, Bafedile Dorcas
dc.date 2018
dc.date.accessioned 2018-10-23T14:15:05Z
dc.date.available 2018-10-23T14:15:05Z
dc.date.issued 2018-09-21
dc.identifier.citation Tlhapi, Bafedile Dorcas (2018) Isolation and structure elucidation of bioctive compounds from Rauvolfia Caffra Sond, University of Venda, Thohoyandou, <http://hdl.handle.net/11602/1259>
dc.identifier.uri http://hdl.handle.net/11602/1259
dc.description MSc (Chemistry)
dc.description Department of Chemistry
dc.description.abstract Rauvolfia caffra Sond, a species of evergreen trees and shrubs in the dogbane family, (Apocynaceae), is used as a medicinal plant among traditional communities in many countries for the treatment of malaria, diabetes, coughs, gastrointestinal disturbances, skin infections, impotence, insomnia, diarrhoea, dysentery, scabies, worm infections, and both parasitic and microbial infections. Phytochemical studies have revealed that indole alkaloids are the major constituents of the stem bark. However, there are limited studies linking the compounds with the ethnomedicinal uses. The aim of this study is to isolate and characterize bioactive compounds from Rauvolfia caffra Sond. The highest phenolic content found in a fraction was 16.06±0.125 mg GAE/g, while the highest flavonoid content measured was 9.453±0.081 mg QE/g. In the DPPH free radical scavenging activity and reducing power tests, a lowest IC50 value of 0.022±0.003 μg/mL and IC0.5 value 0.518±0.044 μg/mL, respectively, was found. Six compounds were isolated from the stem bark, including lupeol, a pentacyclic tri-terpenoid isolated for the first time from the genus Rauvolfia; raucaffricine, a rare glycoalkaloid of the monoterpenoid indole class; N-methylsarpagine, an indole alkaloid isolated for the time from R. caffra and spegatrine, an indole alkaloid isolated for the first time from R. caffra, respectively. Concerning antimicrobial activity, the highest activity of a fraction was against B. cereus with MIC values as low as 12.5 mg/mL. One fraction at the tested concentration (250 μg/mL) decreased the viability of Plasmodium falciparum (4.149±6.979 %) with an IC50 value of 6.533 μg/mL. The crude extract and some fractions affected the viability of the Trypanosomes at the tested concentration (250 μg/mL), giving -0.133 ± 0.206 %, 11.334 ± 2.692 %, 1.026 ± 0.143 % and 20.769 ± 9.054 % with IC50 values of 18.50 μg/mL, 14.15 μg/mL, 15.58 μg/mL and 34.71 μg/mL, respectively. Furthermore, the fractions did not show significant cytotoxic effects at a concentration of 50 μg/mL. en_US
dc.description.sponsorship NRF en_US
dc.language.iso en en_US
dc.rights University of Venda
dc.subject Bioactive compounds en_US
dc.subject Polyphenolic content en_US
dc.subject Rauvolfia Caffra Sond en_US
dc.subject Antioxidant en_US
dc.subject Antimicrobial en_US
dc.subject Antiplasmodial en_US
dc.subject Antiplasmodial en_US
dc.subject Antitrypanosomal en_US
dc.subject Cytotoxic activities en_US
dc.title Isolation and structure elucidation of bioctive compounds from Rauvolfia Caffra Sond en_US
dc.type Dissertation en_US

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